Table 1.
Expression of cell surface antigens and secreted proteins in BS cells and BMSCs
| Antigen | Manufacturer | Label | Marker specification | Positive cells (%) | |
|---|---|---|---|---|---|
| BS cells | BMSC | ||||
| CD34 | Beckman Coulter | PE | Hematopoietic stem cell marker, cell adhesion | 7.69 (+) | 1.05 (–) |
| CD53 | AbD Serotec | FITC | Osteoblast and osteoclast signal transduction | 0.00 (–) | 0.14 (–) |
| CD73 | BD Biosciences | PE | Mesenchymal, epithelial and endothelial cell marker | 97.46 (+++) | 98.67 (+++) |
| CD90 | Beckman Coulter | PE | Fibroblast, stromal and hematopoietic stem cell marker | 95.18 (+++) | 98.37 (+++) |
| CD105 | Beckman Coulter | PE | Mesenchymal and erythroid progenitor cells | 98.05 (+++) | 99.10 (+++) |
| CD106 | AbD Serotec | FITC | Cell adhesion | 4.09 (–) | 55.55 (++) |
| CD133 | Beckman Coulter | PE | Hematopoietic stem cell marker | 0.14 (–) | 1.20 (–) |
| CD144 | AbD Serotec | FITC | Endothelial cells, cell adhesion | 0.01 (–) | 0.58 (–) |
| CD166 | BD Biosciences | PE | Mesenchymal, epithelial stem cells, fibroblasts, monocytes, cell adhesion | 88.06 (++) | 98.42 (+++) |
| ALP | R&D Systems | APC | Alkaline phosphatase | 22.79 (+) | 55.50 (++) |
| FGF | R&D Systems | FITC | Fibroblast growth factor | 45.06 (+) | 42.64 (+) |
| Stro1 | Dako | FITC | Mesenchymal stem cell marker | 0.15 (–) | 0.68 (–) |
+++ marker expression on >95 % of the cells, ++ marker expression on 50-95 % of the cells, + marker expression on 5-50 % of the cells, - marker expression on <5 % of the cells
ALP alkaline phosphatase, APC allophycocyanin, BMSC bone marrow-derived MSC, BS bursa subacromialis, CD cluster of differentiation, FGF fibroblast growth factor, FITC fluorescein isothiocyanate, MSC mesenchymal stem cells, PE phycoerythrin