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. Author manuscript; available in PMC: 2015 Jun 24.
Published in final edited form as: Nat Immunol. 2014 Aug 31;15(10):938–946. doi: 10.1038/ni.2984

Figure 5. Depletion of neutrophils impaired effector lung macrophage polarization after primary Nb inoculation.

Figure 5

(a) Macrophages were electronically sorted from lung of naive mice or mice at day 7 after Nb inoculation treatment with αLy6G or isotype control (day -1, 3). Gene expression for characteristic M1 and M2 macrophage markers and integrins were analyzed by qPCR. Data shown are the mean from a pool of five mice per group (expressed as fold increase over mRNA from lungs of naive mice) and are representative of two independent experiments. (b) Flow sorted macrophages from WT mice at day 7 post infection were seeded to 24-well plates and co-cultured with exsheathed L3 larvae, with or without the presence of anti-CD11b antibody at different concentration. At day 5, larval adherence, larval ATP concentration, and percent mortality were assessed. Mean and SEM of triplicate samples obtained from pools of 5 mice per treatment group. This experiment was repeated three times with similar results. *p<0.01