Skip to main content
. Author manuscript; available in PMC: 2015 Jun 24.
Published in final edited form as: Nat Immunol. 2014 Aug 31;15(10):938–946. doi: 10.1038/ni.2984

Figure 7. Nb-primed neutrophils polarize to an alternatively activated neutrophil phenotype that promotes M2 macrophage differentiation in vitro.

Figure 7

(a) Gene expression of characteristic immune response markers, identified by microarray, from sorted neutrophils (CD11b+Ly-6G+) analyzed by qPCR. Data shown are the mean of triplicate samples from a pool of five mice per group (expressed as fold increase over mRNA from neutrophils of naive mice) and are representative of two independent experiments. (b) Bone marrow derived macrophages (BMDM) were cultured with neutrophils from the lung of mice infected with Nb or treated with LPS. The neutrophil culture was separated from macrophages by transwell in some group, and anti-IL-13 antibody (10 μg /ml) was also added to the culture in some groups. At day 3, Arg1, Chi3l3, Retnla, and Itgam gene expression was determined by qPCR. Gene expression is presented as the ratio of treated/BMDM group without neutrophils added. Data shown are the mean and SEM of triplicate samples and repeated three times with similar results. *p<0.05, **p<0.01.