Figure 6.
Recombinant hTF constructs. Schematic representing recombinant hTF constructs. Mutations introduced into hTF to prevent iron binding in a lobe (as in the case of the FeNhTF, FeChTF and apohTF constructs) or prevent iron release from one lobe (as in the case of LockNhTF and LockChTF) are indicated. All constructs containing an N-terminal hexahistidine tag are non-glycosylated and have all been previously described (Byrne, Chasteen, et al., 2010; Mason et al., 2004). For color version of this figure, the reader is referred to the online version of this book.