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. 2015 Jun 24;11:36. doi: 10.1186/s12990-015-0039-9

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© Tazawa et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Double immunostaining of DβH and pCREB in representative bilateral LC tissues. a–c Naïve rat, contralateral side; j–l naïve rat, ipsilateral side; d–f SNL rat, contralateral side; m–o SNL rat, ipsilateral side; g–i SNL+SCS rat, contralateral side; p–r SNL+SCS rat, ipsilateral side. s Quantitative summary of pCREB-positive neurons in bilateral LC regions. No significant difference in the number of pCREB-positive neurons was noted among the groups. t Quantitative summary of the ipsilateral-to-contralateral ratio of pCREB-positive neurons and DβH immunoreactivity in bilateral LC regions. Quantitative data were obtained from three consecutive slices of four animals per group. Data are mean ± SEM; *p < 0.05 and **p < 0.01 by the Tukey–Kramer test.