Table 6.
Primers used in RT-qPCR for validation of the expression profile obtained by 454 sequencing.
| Clustera | Gene abbreviationb | Gene description | Transcript name | At Locus | Primer sequences (forward / reverse) |
|---|---|---|---|---|---|
| A | MRP10, ABCC14 | ABC transporter C family member 14 | Contig3296 | AT3G62700 | GCTGCCTTTGCCCCACACT/ TGGAAGAGCCTTGAACGCTGCC |
| A | CPK10, CDPK1 | Calcium-dependent protein kinase | Contig4133 | AT1G18890 | GCTCACCTCCCTCGCACAACT/ AGGCGCGAGGTGGCGATAAT |
| B | ASPG1 | Protein aspartic protease in guard cell | Contig19387 | AT3G18490 | ATGCAGCTCGCTCGACGTGT/ TGTCGTGTCCGCAACCCAGA |
| B | ABF2 | Abscisic acid responsive elements-binding factor 2 | Contig22999 | AT1G45249 | CGGCCTGCTTTTTCCGCAACT/ AGTCAGCTGCAAGGTCACGAGC |
| B | AVP1 | Pyrophosphate-energized vacuolar membrane proton pump | Contig 8793 | AT1G15690 | CGCACTTGAGAACGACGCT/ TGCGCGGTCGTCGGAATCAT |
| B | HSP90-7 | Chaperone protein htpG family protein | Qs-dev_rep_c72408 | AT4G24190 | CAGCATCAGCTTCAGCCTCGGT/ AGCGGCTTCACGCCTTCTGA |
| B | BAM1, BMY7, TR-BAMY | β-amylase 1 | Contig 25491 | AT3G23920 | AACGCGAGTTTGCAGGCGCT/ CGACGTTTCCGCCGCATTGA |
| B | GolS2 | Galactinol synthase 2 | Contig 17943 | AT1G56600 | TGACGCCATAACATGGCCAGCA/ TTGCCAGCAGTGCCCTGACA |
| C | ALDH7B4 | Aldehyde dehydrogenase 7B4 | Contig 9124 | AT1G54100 | AGGGTGCTCCAACGACTCCA/ ACGCACAGCTAGGCCGATG |
| C | OPR3 | Oxophytodienoate-reductase 3 | Contig 16616 | AT2G06050 | TTGGTCTCTAACACAGCCGCCG/ CACGGCCGACATGCCATA |
| C | PAL1 | Phenylalanine ammonia-lyase 1 | Contig 9321 | AT2G37040 | TGCTAACTGGCCGCCCCAAT/ GCCAGAACCAACAGCTGTGCCA |
| D | ABI3, SIS10 | Abscisic acid insensitive 3 | Contig 19318 | AT3G24650 | GCAGTGGCCGTGGTGCAAT/ ATGGCGAGGCAAAGGCGGTT |
| D | PHS1 | Glycosyl transferase, family 35 | Contig 10143 | AT3G29320 | TGGCTTGAGATGGGCAACCCT/ TTTGGTGTGCTCCCCGGCAT |
| D | SIR | Sulfite reductase | Contig 19883 | AT5G04590 | TGCAATGGCATGCCCAGCCT/ ACTGGGGTTTCCACCAAGCCA |
| D | PIP2B | Aquaporin PIP2-4 | Contig 24062 | AT2G37170 | GCTTGGGCCTTTGGTGGCA/ TGGCTCCACCACCGTACTTGCT |
| F | COR414-TM1 | Cold-regulated 314 inner membrane 1 | Contig 19349 | AT1G29395 | TGGTGAGCTTGAGCTGCCGT/ TGCAAGCTGCCTGCTCGTGA |
| F | DI21 | Late embryogenesis abundant protein | Contig 16865 | AT4G15910 | AGCGTGCTCTCACCTTGTGGT/ TGCGGCTGCATCACATGCCT |
| F | JMT | Jasmonic acid carboxyl methyltransf. | Contig 17776 | AT1G19640 | TGGCTATCTTCGCCGCCCTT/ TGCCGTGGTTTGCACCTACTTCG |
| F | GSTU25 | Glutathione S-transferase TAU 25 | Contig 17981 | AT1G17180 | ACCACCTCGTCCGCCATTGT/ AGCCTGACCGACTCCATGGCAA |
| F | LTP3 | Lipid transfer protein 3 | Contig 19993 | AT5G59320 | TGCCCAGGCCACCATAACATGC/ TGTGGTCTTGGCCATGCCGTT |
a According to their expression profiles each transcript belongs to a different cluster
b Corresponds to the homologous in Arabidopsis thaliana