Fig. 5.

Decreased VLDL-TG production in REGN1500-treated WT mice. A: WT mice were synchronized for 3 days (fasting: 5:00 PM to 7:00 AM and feeding: 7:00 AM to 5:00 PM). On day 4, mice were refed at 7:00 AM and injected at 9:00 AM with either a control antibody or REGN1500 (10 mg/kg) (n = 5 male mice per group, 10 weeks old). Two hours after the injection, Triton WR1339 (500 mg/kg) was injected into the tail vein. Blood samples were drawn from the tail veins at the indicated time points and then the mice were euthanized and blood was collected. Plasma TG levels were measured enzymatically. Plasma collected at the 90 min time point was pooled and fractionated by FPLC as described in the Materials and Methods, and TG and cholesterol were measured enzymatically in each fraction. The total TG and cholesterol content of the pooled VLDL fractions (7, 8, and 9) is shown below. B: ApoB-100 and ApoB-48 protein levels in the pooled VLDL fractions (7, 8, and 9) were assayed by immunoblotting (top) and quantified using ImageJ. ***, P < 0.001.