Fig. 7.

Hepatic de novo lipogenesis and fatty acid oxidation did not change after ANGPTL3 depletion. A: mRNA levels of genes involved in fatty acid synthesis or oxidation. Livers were collected from refed mice used in the experiment shown in supplementary Fig. 2A. Total RNA was extracted and subjected to whole transcriptome shotgun sequencing using an Illumina 2500 Hi-Seq as described previously (27). B: Rates of de novo lipogenesis in livers and kidneys of Angptl3^−/− mice and littermate controls (n = 8 males per group, 12–16 weeks old). Mice were synchronized for 5 days. De novo lipogenesis was measured at the end of the refeeding cycle as described in the Materials and Methods. C: Fatty acid oxidation in primary hepatocytes isolated from REGN1500- or control antibody-treated WT mice (n = 4 males per group, 12 weeks old). Mice were treated with REGN1500 or control antibody (10 mg/kg) for 4 days. Primary hepatocytes were isolated and oxygen consumption rates were measured using a Seahorse XF analyzer as described in the Materials and Methods.