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. 2015 Jul;56(7):1318–1328. doi: 10.1194/jlr.M056150

Fig. 1.

Fig. 1.

Lp(a) upregulates ABCA1 expression in HepG2 cells. HepG2 cells were treated with 1, 5, and 10 μg/ml purified Lp(a) or LDL protein for 12 h at 37°C. A: ABCA1 mRNA levels after treatment with Lp(a) as determined by RT-PCR. ABCA1 mRNA levels were normalized against β2-microglobulin and GAPDH mRNA and expressed relative to those of the control untreated cells. B: ABCA1 protein levels after treatment with Lp(a) as determined by Western blot. ABCA1 protein levels were normalized against actin (inset) and expressed relative to those of untreated cells. C: ABCA1 protein levels after treatment with LDL as determined by Western blot. ABCA1 protein levels were normalized against actin (inset) and expressed relative to those of untreated cells. D: MDA concentrations in 10 μg/ml Lp(a) protein and 10 μg/ml LDL protein as measured with the TBARS assay. E: MDA concentrations in 10 μg/ml Lp(a) protein and 10 μg/ml LDL protein after the addition of BHT/EDTA in the purification process as measured with the TBARS assay. Results are expressed as mean ± SE of at least two experiments performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared with control.