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. 2015 Jul;56(7):1318–1328. doi: 10.1194/jlr.M056150

Fig. 5.

Fig. 5.

SR-B1 is involved in ABCA1 upregulation. HepG2 cells treated with 5 μg/ml Lp(a) protein were preincubated with an anti-SR-B1 antibody or the Lp(a) protein used for treatment preincubated with an anti-apo(a) polyclonal antibody and the effect on ABCA1, LXRα, and PPARγ mRNA transcript and protein levels determined. Transcript levels were normalized to β2-microglobulin and GAPDH and protein levels normalized to actin with all results expressed relative to the untreated control ABCA1 mRNA levels (A), ABCA1 protein levels (B), PPARγ mRNA levels (C), PPARγ protein levels (D), LXRα mRNA levels (E), and LXRα protein levels (F). Results are expressed as mean ± SE for two experiments performed in triplicate for RT-PCR and triplicate Western blots for protein quantification. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared with control.