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Estimation of bSP-C concentration in surfactant drugs. A: Chromatograms of SRM channels as in Fig. 5B, using Surfacten with or without depalmitoylation procedures. B: Chromatograms of SRM channels for detection of nonpalmitoylated bSP-C, after depalmitoylation of surfactant drugs. C: Standard curve of synthetic bSP-C with nonlinear regression. The peak areas are the sums of the three channels as in (B). D: Estimation of total SP-C concentration in surfactant drugs.
