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. 2015 Jun 24;10(6):e0130060. doi: 10.1371/journal.pone.0130060

Fig 5. MIR34B/C gene analysis in prostate cells and human tissues.

Fig 5

A) Schematic diagram of human MIR34B/C locus on chromosome 11q. B, C) miR-34b-3p and miR-34c-5p expression in the indicated samples of normal prostate (pool of 10 specimens), benign hyperplasia (BPH), prostate cancer (PCa) or non-neoplastic (RWPE-1), hyperplastic (BPH-1) or tumor (LNCaP, DU145) prostate cell lines. D, E) Copy number of genomic DNA regions proximal to MIR34B/C locus (CNV#1; Hs00608392), or to the BTG4 gene (CNV#2, Hs03049129) was evaluated by qPCR in the same samples described in panels B, C. Cutoff values for significant loss or gain of targets DNA relative to a reference assay (RNase P) were defined as copies <0.5 or >4, respectively (no change: CNV = 2). F, G) Analysis of MIR34B/C CpG island epigenetic status was performed by methylation specific PCR in matched normal or neoplastic (PCa) prostate parenchyma, benign hyperplastic samples and prostate cell lines. A sample was considered partially methylated (M/UM) if amplified by both primer pairs. M, Methylated; UM, Unmethylated. Blank, no template control; NA, sample not available.