Fig 4. Distribution and persistence of cocktail phages in grapevines.
(A–F) Distribution of individual cocktail phage (color coded to left) in grapevines was determined at week 2 (A), 4 (B), 6 (C), 8 (D), 10 (E) and 12 (F) post-inoculation. POI (indicated with arrows) and grapevine segments numbered as POI (0), below (-) or above (+) in ~13 cm segments. Root divided into three segments and numbered as R0, R-1, or R-2. Segments of similar proximal distance from the graft point from triplicate grapevines were assayed using qRT-PCR to determine the mean PFU/gt ± s.d. Each bar represents s.d. Standard qRT-PCR line plots obtained for cocktail phage Sano, Salvo, Prado and Paz had R2 values of greater than 0.9 and efficiencies of 129%, 120%, 127% and 123%, respectively.