Fig 5. Combined AE788/Celecoxib treatment reduces the migratory capacity of colorectal cancer cells.

A) Scratch wound healing assay was used to analyze the inhibition of cell migration in colorectal cancer cells treated for 24 h with AEE788 (2.5 µM) and/or celecoxib (10 µM). Data are means ± SEM of three independent experiments (*p <0.05, compared with the control; # p<0.05, compared with AEE788-treated cells). Final magnification: X100, scale bar corresponds to 100 microns. B) Representative images of scratched areas in confluent Caco-2 and HCT-116 cell layers. The yellow lines indicate the invasive front in the wound healing assay. Wound closure was photographed at 0h and 24 h after wounding. The scratched area at control (0 h) was arbitrarily assigned as 100%.