Table 1. List of primers used for PCR.

S. No.	GENE	FORWARD PRIMER 5’-3’ sequence	REVERSE PRIMER 5’-3’ sequence	DETAILS
1	αSMA	CAGCCAAGCACTGTCAGG	CAATGGATGGGAAAACAGC	150 bp, 59.5°C
2	γSMA	CCTCAGTCACTGGGAGAAGAA	ATCATCTCCTGCGAAGCCT	150 bp, 59.5°C
3	BMP1	ACAGCACAGGCAACTTCTCC	GGGACGTGAAGTTCAGGATG	117 bp, 59.5°C
4	CD248	TGCCAACGTGTGTCTTTTTG	GTTCTGTTGGGCTCTTGCTC	141 bp, 59.5°C
5	COL15A1	CAGTGCTGGTGTCTGCTGAT	GACAAAGGATACGGACGAGG	150 bp, 59.5°C
6	CTGF	CAGCATGGACGTTCGTCTG	CCAACCACGGTTTGGTCCTT	117 bp, 59.5°C
7	EDN1	CGAGCACGTTGTTCCGTATG	CAGCCCTGAGTTCTTTTCCTG	164 bp,55°C
8	EGR2	GTGACCATCTTTCCCAATGC	AGCAAAGCTGCTGGGATATG	135 bp, 62°C
9	ELN	GTCCTCCTGCTCCTGCTGT	CTCCTCCTCCAAGGGCTC	127 bp, 62°C
10	FN1	AAACCAATTCTTGGAGCAGG	CCATAAAGGGCAACCAAGAG	142 bp, 50°C
11	GATA6	TGCAGCAAAAATACTTCCCC	TGTAGAGCCCATCTTGACCC	133 bp, 62°C
12	IGF2	GCTTCCAGACACCAATGGGAATCC	TCATATTGGAAGAACTTGCCCACG	364 bp, 60°C
13	IGFBP3	AGAGCACAGATACCCAGAACT	TGAGGAACTTCAGGTGATTCAGT	105 bp, 59.5°C
14	INHBB	GCGTTTCCGAAATCATCAG	TTTCAGGTAAAGCCACAGGC	134 bp, 59.5°C
15	LIMK1	GGAGAGGAAGGAAGCGAGTT	TAGTACTGGTGCGACAGGGA	146 bp, 59.5°C
16	PLOD2	GGACTCGGAGAAGCCCTC	CCTTGACCAAGGACCTTCAC	138 bp, 59.5°C
17	PTN	TGCAACAAAGGCAGACTGAG	TCCCTGCTTCAGCAGTATCC	148 bp, 59.5°C
18	RPL35A	GAACCAAAGGGAGCACACAG	CAATGGCCTTAGCAGGAAGA	236 bp, 58°C
19	TAGLN	GCTCTACTGTCTGTTGCCCC	CCTCCAGCTCCTCGTCATAC	135 bp, 62°C
20	TGFβ2	AGTGCCTGAACAACGGAT	GTACAAAAGTGCAGCAGG	218 bp, 55°C
22	TGM2	TGACCTCCGCAAAGACAAAG	CCAAGTTGCGGAAGCAGTA	241 bp,50°C
23	THBS1	CCGGCGTGAAGTGTACTAGCTA	TGCACTTGGCGTTCTTGTT	317 bp, 59°C
24	TGFBI	TGTGTGCTGAAGCCATCGTTG	CCGGCTTGTCTGAAAAGGTCA	313 bp,50°C
25	TMEPAI	TTCATTCCCTGTCCTCATTGG	GCACAACAGCCATGGAATCA	228 bp, 50°C
26	TGFβ1	TCCGAGAAGCGGTACCTGAA	TGCTGTCACAGGAGCAGTGG	266 bp,63.7°C
27	TGFβ3	GCGTGAGTGGCTGTTGAGA	CCAAGTTGCGGAAGCAGTA	306 bp,52.7°C
28	TGFβRI	TACAGCTTTGCCTGAACTCT	CACGACAGAGTTACCTAAAG	311 bp,52.7°C
29	TGFβRII	AGTGTTGGGTTATTGCTAAT	AGTGACTTCACAATGTAAAC	240 bp,54.3°C
30	TGFβRIII	ATTCTTTTCAGGCCAGTGGC	TGGAACCTGTATCACAATGGAG	182 bp,63.2°C