. Author manuscript; available in PMC: 2016 Apr 1.

Published in final edited form as: Future Med Chem. 2015 Jun;7(8):989–1007. doi: 10.4155/fmc.15.42

Table 2.

Assays for testing SecA inhibition.

No.	Assay	Information	Ref.
1	ATPase assay	Investigates the activity of SecA on ATP hydrolysis	[126–128]
2	SecA-LacZ reporter assay	Detects SecA inhibitors by investigating expression of SecA inhibition due to autogenous regulation	[129]
3	Antisense-mRNA assay	Identifies SecA inhibitors by using antisense-mRNA to block SecA translation and causing bacterial sensitization	[124]
4	Protein translocation assay	Examines the protein transport activity of SecA through vesicles and liposomes	[130–132]
5	Protein ion-channel activity assay	Measures the ion-channel activity of SecA	[113,133]