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. 2015 Apr 3;145(3):347–356. doi: 10.1111/imm.12444

Figure 2.

Figure 2

In vivo differentiation of T helper type 17 (Th17) cells was blocked by administration of TMP778. Mice were immunized with myelin oligodendrocyte glycoprotein peptide 35–55 (MOG35–55) in complete Freund's adjuvant (CFA). TMP778 (20 mg/kg or 5 mg/kg) or Vehicle was subcutaneously injected twice a day for 6 days. (a) The draining lymph node cells were isolated and stimulated with MOG35–55 for 5 days and the supernatants were harvested to detect interleukin-17 (IL-17) titres by Meso Scale Discovery. (b) Draining lymph node cells were isolated and stimulated with MOG35–55 for 20 hr. Cells were harvested for RNA extraction and real time RT-PCR analysis for mRNA expression of Il17a, Il17f and Il22. *P < 0·05 TMP778 versus Vehicle; **P < 0·01 TMP778 versus Vehicle. Data are representative of three separate experiments.