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. 2015 Apr 3;145(3):347–356. doi: 10.1111/imm.12444

Figure 3.

Figure 3

Retinoic acid receptor-related orphan nuclear receptor γt (RORγt) inverse agonist inhibits the generation of interleukin-17 (IL-17) -producing CD4+ T cells in an IL-17/GFP transgenic model. (a) IL-17/GFP expression from naive lymph nodes. (b) IL-17-IRES-GFP transgenic mice were immunized with myelin oligodendrocyte glycoprotein peptide 35–55 (MOG35–55) in complete Freund's adjuvant (CFA). TMP778 (20 mg/kg) or Vehicle was subcutaneously injected twice a day for 9 days. The draining lymph node cells were purified and stimulated with PMA/ionomycin in the presence of brefeldin A for 3 hr before staining for mouse CD4. (c) Average of integrated MFI (iMFI) of individual animal CD4+ IL-17/GFP+ quadrant, as in Figure4(b). *P < 0·05 TMP778 20 mg/kg versus Vehicle. Data are representative of two separate experiments.