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. 2015 May 19;145(3):367–379. doi: 10.1111/imm.12449

Figure 1.

Figure 1

Osteoclasts express numerous ligands for both activating and inhibitory natural killer (NK) cell receptors. Osteoclasts were surface-stained for expression of ligands for multiple NK cell receptors after enrichment. (a), Ligands for activating receptor NKG2D: MHC class I-chain related protein A (MIC-A), MIC-B, UL16-binding protein 1 (ULBP-1), ULBP-2/5/6 and ULBP-3. (b) Ligands for activating receptors DNAX accessory molecule 1 (DNAM-1):CD155 and CD112; ligand for 2B4 (CD244):CD48; and ligand for leucocyte function-associated antigen 1 (LFA-1): intercellular adhesion molecule (ICAM-1) (CD54). (c) Ligands for tumour necrosis factor-related apoptosis-inducing ligand (TRAIL): death receptor 4 (DR4) and DR5; ligand for Fas ligand (FasL): Fas (CD95). (d) Ligands for inhibitory NK cell receptors: HLA-ABC and HLA-E. Open histograms represent indicated antibody and filled histograms represent isotype-matched control immunoglobulin. The histograms are representative of n = 3 to n = 6 donors. The median fluorescence intensity (MFI) of all donors is represented in the dot plots under each histogram. Two-tailed, paired Student's t-test; *P < 0·05; **P < 0·01; ***P < 0·001; ****P < 0·0001.