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. Author manuscript; available in PMC: 2015 Dec 23.
Published in final edited form as: Nat Commun. 2015 Jun 23;6:7476. doi: 10.1038/ncomms8476

Figure 1. LPGDS is a downstream target of Zic1.

Figure 1

(a) Experimental design for the selection of Zic1 targets. (b) Fold induction of Six1, Eya1 and (c) LPGDS from the microarray data. (d) In situ hybridization for LPGDS (stages 13, 14 and 16 are frontal views; stage 15 and stage 27 are lateral views, anterior to right, dorsal to top). (e) In situ hybridization for LPGDS and Zic1 in stage matched embryos (upper panels). LPGDS and Zic1 co-localize at the anterior neural plate (arrowheads), while Zic1 is also expressed in neural crest progenitors (arrows). Double in situ hybridization (lower panels) shows overlapping expression of LPGDS and Zic1 at the anterior neural plate (arrowheads; left panel), while LPGDS and Snail2 (right panel) have adjacent but non-overlapping expression domains (black arrowheads). Frontal views. (f) In embryos injected with Zic1GR mRNA and treated with dexamethasone (+Dex), LPGDS is dramatically expanded (arrowhead), while Foxi1c and Six1 expression at the PPR are reduced (arrowheads). The same injection in the absence of dexamethasone (-Dex) had no effect on the expression of these genes Frontal views, the injected side is indicated by the lineage tracer (Red-Gal). (g) Quantification of the Zic1GR injection results. Three independent experiments were performed. The number of embryos analyzed (n) is indicated on the top of each bar. (h) Zic1 knockdown (Zic1-MO injection) reduces LPGDS, Foxi1c and Six1 expression. (i) Quantification of the Zic1-MO injection results. Three independent experiments were performed. The number of embryos analyzed (n) is indicated on the top of each bar. Scale bars, 200 μm.