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. Author manuscript; available in PMC: 2015 Dec 23.
Published in final edited form as: Nat Commun. 2015 Jun 23;6:7476. doi: 10.1038/ncomms8476

Figure 3. Retinoic acid affects PPR formation in a dose dependent manner.

Figure 3

(a) Treatment of intact embryos at stage 11 with increasing doses of RA (0.01 μM, 0.1 μM, 1 μM and 10 μM) disrupts Fox1ic and Six1 expression at the PPR. DMSO was used as a control. Frontal views, dorsal to top. (b) Quantification of the results. Four independent experiments were performed. The number of embryos analyzed for each condition (n) is indicated on the top of each bar. Scale bars, 200 μm.