Table 3. The best and lowest similarity thresholds for taxonomic resolution.

Sequences	V regions	The best similarity thresholds/success (%)				The lowest similarity thresholds/accuracy (%)*
		S/G	G/F	F/O	O/I	Intra-species	Intra-genus	Intra-family	Intra-order
Nearly-whole-length	V1-9	99.9/93	98.4/64	94.2/86	90.2/81	100/97	100/100	97/96	93/96
Section 1	V1-3		99.4/63	95.4/82	90.6/82			98/95	93/96
Section 2	V4-5	99.7/66	97.8/67	92.6/83	88.6/78		100/99	97/96	92/95
Section 3	V7	99.3/82	94.6/73	89.9/68	86.0/68		100/96	96/97	91/96
Section 4	V8		99.8/62	95.7/89	90.6/68			98/96	95/97
Section 5	V9	99.3/67	96.6/79	90.2/78	83.2/77		100/95	96/96	90/96

* 95% is the lowest accuracy accepted in this case.