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. 2015 May 6;4(7):852–861. doi: 10.5966/sctm.2014-0111

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Figure 1. — In vitro LIVE/DEAD assay of EPCs-MSCs cocultures exposed to 24 hours of LPS. EPCs were coembedded in hyaluronic acid-based (HA)-hydrogels with nonhypoxic (A, B) or hypoxic preconditioned (C, D) MSCs and exposed to 24 hours of LPS of varying concentrations. Subsequent cellular vitality was quantified (A, C) using a LIVE/DEAD assay in which live cells stain with calcein (green) and dead cells with ethidium homodimer (red) (B, D). Coembedding EPCs with MSCs in HA-hydrogels (three-dimensional culturing) conferred improved protection against increasing LPS concentrations (compared with two-dimensional coculturing of EPCs with MSCs on the surface of culture dishes instead of embedded in HA-hydrogels). Hypoxic preconditioning of MSCs improved stem cell viability in nonembedded cells. n = 6. ∗, p ≤ .05 vs. embedded. Magnification, ×150. Scale bar = 100 µm. Abbreviations: EPCs, endothelial progenitor cells; (h), hypoxic; LPS, lipopolysaccharide; MSCs, mesenchymal stem cells.