Figure 2.

Colony areas and numbers during culture. Human induced pluripotent stem cell (hiPSC) lines or Tic or iPS-TIG114-4f1 cells were seeded using feeder-free culture conditions in 6-well-plates and cultured for 5 days. After the cell clumps had settled on the plate surfaces (48 hours), phase-contrast images of these cells were acquired every 12 hours and analyzed using the culture observation system and software. Cell clumps areas of >25 μm² were recognized as undifferentiated hiPSC colonies for analysis. (A): Colony areas for Tic feeder-free cell culture. (B): Total colony numbers for Tic feeder-free cell culture. (C): Colony areas for iPS-TIG114-4f1 feeder-free cell culture. (D): Total colony numbers for iPS-TIG114-4f1 feeder-free cell culture. The plots are representative of three wells from independent experiments. Abbreviation: iPS, induced pluripotent stem.