Figure 1.

Implementation and characterization of a standardized protocol for hESC expansion. Schematic representation of the hESC expansion bioprocess herein developed. Two hESC lines, a feeder-dependent line (hESC-C, in green) and a feeder-free line (hESC-M, in red), were expanded under fully defined conditions using stirred-tank bioreactors and characterized by transcriptomic and metabolic tools toward implementation of a standardized and robust protocol for hESC expansion. The images were acquired using magnification ×100. Abbreviation: hESC, human embryonic stem cell.