Figure 2.

Expression profiles of EM ASCs resemble human brown/beige fat. (A): Undifferentiated ASCs of the indicated origin are plotted for their expression of the brown fat transcriptional regulator PRDM16. Expression of white (B), beige (C), and brown (D) genes is plotted for differentiated ASCs. (E): Gene expression for those markers in B–D was also evaluated and internally normalized (EM/s.c.) for patient-matched samples. (F): Immunofluorescent images of UCP1 protein qualitatively reflect gene expression data with brighter signal in EM ASC (intact) cultures. ∗, p < .05; ∗∗, p < .01; ∗∗∗, p < .001. Abbreviations: ASCs, adipose-derived stem cells; EM, epimuscular; UCP1, uncoupling protein 1.