Figure 7.

GR silencing attenuated MA-induced adipogenic differentiation. (A): siGR significantly attenuated MA- and Dex-induced Oil Red O staining and lipid accumulation in adipogenic induction medium. (B): In addition, siGR significantly downregulated MA- and Dex-induced adipogenic marker genes such as ADIPOQ, C/EBP-β, FABP4, and PPAR-γ in quantitative real-time polymerase chain reaction (black bars, control; gray bars, MA treatment; white bars, Dex treatment). ∗∗, p < .01. Abbreviations: GR, glucocorticoid receptor; MA, megestrol acetate; siCON, small interfering control; siGR, small interfering GR.