FIG 1 .

HK2 is important for parasite growth at 3% oxygen. (A) siRNA screen overview. (B) Z scores of siRNA screening data at 21% (x axis) and 3% (y axis) O₂. Dotted lines are Z scores of less than −2 or >2. siRNAs that reduce host cell viability are highlighted in red. (C) HK2 Western blot of lysates from mock- or parasite-infected negative-control (Neg Ctrl) or HK2 siRNA-transfected HeLa cells grown at 21% oxygen. Parasite growth was measured in HIF-1α (D) or HK2 (E) siRNA-transfected cells. Shown are averages and standard deviations from three assays. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (Student’s t test).