FIG 3 .

HK2 expression in HIF-1αKO MEFs restores Toxoplasma growth. (A) Representative Western blots of FLAG-tagged HK1 and HK2 expression in HIF-1αWT MEFs. (B) Parasite growth was measured 72 hpi in HIF-1αWT or HIF-1αKO MEFs transfected with EV, HK2, or eGFP expression constructs. (C) Parasite growth measured 72 hpi in HIF-1αWT or HIF-1αKO MEFs that were mock transfected or transfected with an HK2 or HK1 expression construct. Shown are averages and standard deviations of three experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (Student’s t test).