FIG 5 .

Parasite growth in C2C12 murine myoblasts is HIF-1α and HK2 dependent at physiological O₂ levels. C2C12 cells were transfected with HIF-1α, HK2, or negative-control (Neg.Ctrl.) siRNAs, and 72 h later, target gene expression (A) or relative cell numbers (B) were measured. (C) siRNA-transfected C2C12 cells were infected and parasite growth was determined 72 h later. Shown are the averages and standard deviations of three assays. ns, not significant; *, P < 0.05; **, P < 0.01 (one-way ANOVA).