FIG 6 .

Toxoplasma increases HK2 expression in vivo. (A) Lungs, livers, and spleens were collected from mice 5 days postinfection and Western blotted to detect HK2 (HK2 band denoted by arrow). (B) Quantification of HK2 from three assays. Shown are averages and standard deviations. **, P < 0.01 (Student’s t test). (C) HK2 expression in infected (GFP⁺) and uninfected (GFP⁻) monocytes harvested from peritoneal cavities of intraperitoneally infected mice. The HK2^hi expression gate was set empirically on the basis of fluorescence-activated cell sorter plots. Shown is a representative sample of five infected mice. MFI, mean fluorescence intensity.