FIG 7 .

Toxoplasma promotes mitochondrial dissociation of HK2, but not HK1, at 3% O₂. (A, B) Schematic of HK2 expression constructs (A) and Western blot assays confirming expression (B). (C) Parasite growth was measured 72 hpi in HIF-1αWT or HIF-1αKO MEFs transfected with the HK2 constructs indicated. (D and E) Mitochondrial (lanes M) and cytoplasmic (lanes C) fractions from uninfected or infected HIF-1αWT MEFs (18 hpi) were blotted to detect HK2 (D) or HK1 (E) and succinate dehydrogenase complex subunit A (SDHA), as a mitochondrial marker. Graphs show averages and standard deviations of relative HK2 or HK1 abundance in each fraction from three experiments. *, P < 0.05; **, P < 0.01 (one-way ANOVA). WCL, whole-cell lysate.