FIG 7 .

The effect of strain expression of FHbp on complement-mediated bactericidal activity of affinity-purified human anti-FHbp antibodies. Data are from wild-type strain H44/76 with naturally high FHbp expression (solid line) and from a mutant of strain H44/76 with lower FH expression (dashed line). (A and B) Flow cytometric detection of bound antibody. (A) Murine anti-FHbp MAbs (JAR 5 and JAR 4; 50 µg/ml). (B) Control anti-PorA P1.7 MAb (5 µg/ml). (C and D) Bactericidal activity with human complement. (C) Affinity-purified human anti-FHbp antibody. (D) Control murine anti-PorA P1.7 MAb. Data were replicated in two or three independent experiments.