. 2015 Jun 23;6(3):e00842-15. doi: 10.1128/mBio.00842-15

TABLE 2 .

Summary of kinetic data on binding of human Fabs to FHbp ID 1 as measured by surface plasmon resonance^a

Fab no.^b	k_a × 10⁻⁶ (M⁻¹ s⁻¹)	k_d × 10³ (s⁻ 1)	K_D × 10⁹ (M)	R_max (RU)	χ² (RU²)	GenBank accession no.
Fab no.^b	k_a × 10⁻⁶ (M⁻¹ s⁻¹)	k_d × 10³ (s⁻ 1)	K_D × 10⁹ (M)	R_max (RU)	χ² (RU²)	Light chain	Heavy chain
1A	2.25	0.1	0.44	45	0.02	KP770107	KP770108
2A	0.53	61.0	115	31	0.38	KP770109	KP770110
3C	0.03	3.2	130	11	0.28	KP770111	KP770112
4B	0.72	0.6	0.86	119	0.88	KR017714	KR017716
5B	0.39	4.1	10.6	141	2.1	KP770113	KP770114
6B	0.50	0.4	0.82	98	0.20	KP770115	KP770116
7B	0.18	4.1	22.9	114	0.87	KR017715	KR017717
8A	2.30	2.0	1.04	57	0.02	KP770117	KP770118
9B	1.85	0.04	0.019	58	0.55	KP770119	KP770120
10C	0.93	0.5	0.68	132	0.73	KP770121	KP770122
JAR 5	8.84	0.5	0.054	84	0.14	JF715927	JF715926
Fab 502	1.09	5.3	4.8	105	1.1	EU835941	EU835942

^{^a}

k_a, association rate constant; k_d, dissociation rate constant; K_D, equilibrium dissociation constant; R_max, maximal binding response units (RU); χ², chi-square value indicating quality of fit of data to a 1:1 binding model. χ² was <3% of R_max.

^{^b}

Each of the Fab numbers except JAR 5 and Fab 502 includes a letter (A, B, or C) which corresponds to one of the three subjects. JAR 5 and Fab 502 are recombinant chimeric human-mouse Fabs derived from murine anti-FHbp MAb JAR 5 (20) and MAb 502 (19), respectively.