Fig. 2. Apoptotic cell death was induced by parthenolide (PT) and balsalazide. (A) After treatment with PT and/or balsalazide for 24 h, cells were harvested and stained with annexin-V FITC/propidium iodide (PI). (B) Cell cycle modification induced by combination treatment. After treatment with PT and/or balsalazide for 24 h, cells were harvested, fixed, and stained with PI. The percentage of the population in sub-G1 is reported in each histogram and the total number of events analyzed for each condition was 10,000.
