Fig. 4. Down-regulation of caspases by parthenolide (PT) and balsalazide. HCT116 cell lysates were prepared after treatment with PT, balsalazide, or PT and balsalazide for 24 h and then analyzed by western blotting with caspase-3 or caspase-8 antibodies. Combined treatment reduced the levels of full length caspase-3 and caspase-8. However, caspase-3 and caspase-8 did not change when cells were pre-treated with a pan-caspase inhibitor (Z-VAD-FMK). Actin was used as the loading control.
