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. 2015 Jun 25;5:11620. doi: 10.1038/srep11620

Figure 4. Fibronectin production was increased in TRPC3 overexpressing fibroblasts when they were subjected to repetitive stretching.

Figure 4

TRPC3 overexpressing fibroblasts and control cells were stretched for 24 hours and the expression levels of Fibronectin were assessed using qRT PCR and Western Blot analysis. The blots presented here were cropped to improve clarity. Uncropped, full-length blots are presented in Supplementary Figures S6–10. a; qRT PCR demonstrated that the gene expression level of Fibronectin was significantly increased after 24 hours of stretching in TRPC3 overexpressing cells compared to control cells. Data represents means ± SD of 3 samples. *p < 0.05 by Tukey-Kramer test. b; Immunoblotting data demonstrated that the production of Fibronectin was markedly enhanced when TRPC3 overexpressing cells were stretched for 24 hours (n = 3). c; Increased Fibronectin production in TRPC3 overexpressing cells subjected to 24 hours stretching was attenuated by the addition of Pyr3 (n = 3).