Fig 4. DC subsets are equally efficient in direct antigen presentation.

(A) Mice were injected with ECTV, D-LN harvested and cells stained with antibodies to identify non-NK, non-B, non T cell, GFP⁺ CD11c⁺ DC subsets as: pDC (B220⁺CD11b^-), CD8α (B220^-CD8α ⁺CD11b^-), CD11b⁺ (B220^-CD11b⁺CD8α ^-). Nos. represent % of cells in 3 representative experiments using 3 mice per condition. (B and C) Mice were injected with NP-S-EGFP or NP-EGFP, D-LN harvested and stained as described in (A), with the addition anti- K^b-SIINFEKL. Quantification of K^b-SIINFEKL expression and efficiency was determined as described in Fig 2C. Data are pooled from 3 experiments using 3 mice per condition. (D) Mice were injected with NP-EGFP or NP-S-EGFP, then D-LN cells were FACS sorted for EGFP⁺ or EGFP^- pDC, CD8α ⁺, or CD11b⁺ DC, as above. Each population was co-cultured with OT-I T_CD8+ that were then analyzed for proliferation as above. Data are pooled from 3 experiments, using 15 mice per condition to obtain sufficient cells. (E) As in (A), except for addition of anti-CD80 and anti-CD86 antibodies. Data are pooled from 3 experiments. All graphs show (mean ± standard error), P values *p<0.05, **p<0.01, ***p<0.001, NS (not significant) using Student’s unpaired t-test.