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. 2015 Jun 25;5:11544. doi: 10.1038/srep11544

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(1) Representative images of MCF-7 cells treated with medium alone and compounds (A and B) at 4 and 6 μg/mL, respectively, and stained with Hoechst 33342 for nuclei, cytochrome c, membrane permeability and MMP dyes. Both compounds induced a noteworthy elevation in membrane permeability and cytochrome c release and a marked reduction in mitochondrial membrane potential (magnification: 200×). (2) Representative bar charts indicating dose-dependent increases in cell permeability, reduced MMP and increased cytochrome c release in A- and B-treated MCF-7 cells.