Figure 9. (1) Relative luminescence time-dependent expression of caspases 3/7, -8 and -9.

MCF-7 cells treated with A and B at concentrations of 4 and 6 μg/mL, respectively, after 24 hours of incubation showed significant expression of caspases 3/7, -8 and -9. (2) Western blot analysis of A- and B-treated MCF-7 cells. Cells were treated with compounds for 24 hours before being lysed and subjected to separation by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Proteins were then transferred to a membrane and probed with antibodies against caspase 7, -9 and Bid. The membrane was reprobed with anti-β-actin antibody as the loading control. The band densities of treated samples were normalized to the control. The results revealed significant activation of caspases -7, -9 and Bid.