Figure 3. Blockage level patterns for zif268 + DNA in a thinned nanopore.

a) DNA samples for binding: (−)DNA contains no binding sites and (+)DNA contains one centered binding site (red square), as identified by scoring the sequences according to a zif268 position weight matrix (see Supplementary Information for details, Table S1). b) DNA sample events showing all observed blockage sublevel patterns: A, ABA, AC, ABAC, and C. c) Scatter plots showing event average depth I_B and duration t_D (gray) as compared to sublevel depth I_Bⁱ and duration t_Dⁱ (by color, as indicated on inset sample events). To facilitate visualization of population density, a random white noise offset below the acquisition rate of this data (−2 < ∆t < + 2 μs, acquisition rate 250 kHz) has been added to the duration of each blockage level in the event diagram.