Table 2.
Barcoding information for the four markers investigated here for 355 samples representing 93 Crataegus taxa [see Supporting Information—Table S1]. 1Percentage of individuals successfully sequenced. 2Outgroups excluded; calculated using MEGA v. 6.0 (Tamura et al. 2013).
| Marker | matK | rbcLa | psbA-trnH | ITS2 |
|---|---|---|---|---|
| Aligned sequence length (bp) | 783 | 552 | 429 | 671 |
| Unaligned length (mean); excluding end gaps | 321 (705.7 ± 99.3) 783 | 485 (551.3 ± 5.3) 552 | 183 (274.9 ± 22.0) 429 | 112 (312 ± 92.5) 587 |
| Pairwise % identity | 99.3 | 99.8 | 87.6 | 79.8 |
| Number of taxa successfully amplified and sequences | 82 | 93 | 81 | 65 |
| Number of samples successfully amplified and sequenced | 255 | 340 | 290 | 192 |
| % sequencing success1 | 71.8 | 95.8 | 81.7 | 54.1 |
| Overall mean sequence divergence2 | 0.001 | 0.002 | 0.128 | 0.135 |