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. Author manuscript; available in PMC: 2016 Jul 1.

Published in final edited form as: J Pept Sci. 2015 Mar 24;21(7):554–560. doi: 10.1002/psc.2760

Fig 4 — Comparative analyses of the biological properties of recombinant and synthetic IFNα2b. (A): Antiviral activity of the two molecules was determined based on a visual read of the protection of A549 cells from cytopathic effect (CPE) due to viral challenge with encephalomyocarditis virus (ECMV). Recombinant and synthetic IFNα2b were titrated in duplicate samples, and protection from CPE due to ECMV infection was determined in comparison to untreated A549 cells (no IFNα), and to uninfected, untreated cells (no virus, no IFNα) controls. (B, C): Inhibition of cell proliferation. Peripheral blood mononuclear cells (PBMC) of healthy volunteers were plated in triplicate samples, and proliferation was stimulated with Staphylococcal enterotoxin B and IL-2 (Roche Biochemicals). Cells were cultured in the presence of recombinant or synthetic IFNα2b (0.01–10 ng/ml) for 3 days at 37°C. Proliferation was determined by incorporation of [³H]-Thymidine compared to untreated samples (B). In samples treated with 10 ng/ml IFNα2b, cellular proliferation was also measured by cell counts with trypan blue exclusion (C).

Fig 4 — Comparative analyses of the biological properties of recombinant and synthetic IFNα2b. (A): Antiviral activity of the two molecules was determined based on a visual read of the protection of A549 cells from cytopathic effect (CPE) due to viral challenge with encephalomyocarditis virus (ECMV). Recombinant and synthetic IFNα2b were titrated in duplicate samples, and protection from CPE due to ECMV infection was determined in comparison to untreated A549 cells (no IFNα), and to uninfected, untreated cells (no virus, no IFNα) controls. (B, C): Inhibition of cell proliferation. Peripheral blood mononuclear cells (PBMC) of healthy volunteers were plated in triplicate samples, and proliferation was stimulated with Staphylococcal enterotoxin B and IL-2 (Roche Biochemicals). Cells were cultured in the presence of recombinant or synthetic IFNα2b (0.01–10 ng/ml) for 3 days at 37°C. Proliferation was determined by incorporation of [³H]-Thymidine compared to untreated samples (B). In samples treated with 10 ng/ml IFNα2b, cellular proliferation was also measured by cell counts with trypan blue exclusion (C).