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. Author manuscript; available in PMC: 2016 Sep 24.
Published in final edited form as: Brain Res. 2014 Dec 24;1621:170–186. doi: 10.1016/j.brainres.2014.12.026

Fig. 3.

Fig. 3

α4 and δ expression is increased on the plasma membrane of dendritic shafts of CA1 hippocampal pyramidal cells during proestrus. (A) Representative images of dendritic shafts (sh) in stratum radiatum across the estrous cycle, showing silver-intensified immunogold (SIG) labeling of the GABAR subunits on the plasma membrane (arrowheads) by electron microscopic immunocytochemistry (EM/ICC). Representative images from mice during diestrus (i), proestrus (ii) and estrus (iii). Scale, 500 μm. (B) Bar graph shows mean±SEM of SIG particle density per unit length of plasma membrane. ANOVA, α4, F(2, 177)=29.02, *P<0.0001; δ, F(2, 177)=3.8, *P=0.0121. n=60 pooled from 3 mice/group. (C) Western blot, hippocampal α4 and δ expression in vehicle and E2-treated mice. (D) Mean±SEM, optical density. α4, t(4)=6.2, *P=0.003; δ, t(4)=3.94, *P=0.017, n=9.