Skip to main content
. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Hepatology. 2015 Feb 27;62(1):220–231. doi: 10.1002/hep.27677

Fig. 4. LSD1 is recruited to CYP7A1 and CYP8B1 genes in a SHP-dependent manner and demethylates H3K4-me3, which is required for additional repressive histone modifications in HepG2 cells.

Fig. 4

(A) Effects of CDCA treatment on occupancy of LSD1 and SHP and histone H3K4-me3 levels at the CYP7A1 and CYP8B1 promoters as detected by ChIP. (B) Effects of downregulation of SHP on occupancy of LSD1 and histone H3K4-me3 levels. SHP levels detected by IB are shown at the left. (C) Effects of downregulation of LSD1 on histone H3K4-me3, H3K9-me2, and H3K9/14-Ac levels. Reduced LSD1 levels detected by IB are shown at the left. (D) Effects of downregulation of LSD1 on mRNA levels of CYP7A1 and CYP8B1. The mean and SEM (n=3) are plotted and statistical significance was measured using Student’s t-test. *p<0.05.