Table 1.
Nucleotide sequences of primers and probe used for quantitative real-time PCR experiments
| Gene/accession ID | Nucleotide sequence (5′ to 3′) | Position | Amplicon length | |
|---|---|---|---|---|
|
Rn18s
NR_003278.3 |
Fwd | GCATGGCCGTTCTTAGTTGG | 1330 | 77 bp |
| Rev | TGCCAGAGTCTCGTTCGTTA | 1377 | ||
| Probe | TGGAGCGATTTGTCTGGTTATTCCGA | 1350 | ||
|
Rxfp1
NM_212452.1 |
Fwd | GCTTCCACTAACTCCTTTGAGGC | 247 | 76 bp |
| Rev | CATGCATTGTTTGTGCCGAG | 322 | ||
| Probe | AAACTTCCGAATGCGTGGTTGGCTC | 271 | ||
|
Rln
NM_011272 |
Fwd | AGGCAAGCCACTGAAGTTGT | 273 | 62 bp |
| Rev | GTCGTATCGAAAGGCTCTGC | 315 | ||
| Probe | GCCATCCTTCATCAACAAAGA | 293 | ||