Fig. 2:

HUMS cells expressed- trophic factors and their functionality.

Trophic factor expression- RT-PCR : 1- BDNF, 2- GDNF, 3- NT-3, 4- NGF, 5-VEGF, 6- IGF-1 and 7- CNTF. B-D. Functionality of HUMS cells secreted trophic factors in the CM.

B. Motor neuronal cells, NSC34, are: a. rarely differentiated; b. Neuronal differentiation, extensive long neurite outgrowth and networking upon CM treatment; Arrows- neurite extension; Square –several neurites networking. Scale bar = 100 mm.

C. ~3 fold and ~5 fold neuronal differentiation upon 48 hrs and 96 hrs CM treatment respectively.

D. CM induces extensive long neurite outgrowth. Statistical significance- ** -P&0.001. Error bars represent standard deviation.