Author response image 1.
Viral titers of CVB5 bound to FACS sorted primary rat α or β cells. FACS-purified rat α cells (˃90% purity) were infected with CVB5 (M.O.I. 5). After 2 hr incubation cells are washed with medium 3 times before freezing in complete medium. The cell-bound virus was then titrated by limit dilution assay and TCID50 titers calculated according to the Kärber formula. To determine the background of unbound virus, wells devoid of cells were treated the same way and corresponding titers. The unbound viruses were subtracted in each experiment. Results are Mean and SEM of 2-3 independent experiments.