FACS-purified rat α and β cells (>90% purity) were infected with CVB5 (M.O.I. 5, 50 or 100 for α cells; M.O.I. 5 for β cells) (A), CVB4 (M.O.I. 5) (B), or CVB5 (M.O.I. 5) (C and D) for 36 hr. (A and B) Apoptosis was evaluated by staining with the nuclear dies Hoechst 33342 and PI. Coxsackievirus and adenovirus receptor (CAR) (C) and DAF (D) mRNA expression were assayed by RT-PCR and normalized by the housekeeping gene GAPDH. Results are from 4–8 experiments, plotted as box plots indicating lower quartile, median, and higher quartile, with whiskers representing the range of the remaining data points; *p < 0.05 and ***p < 0.001 CVB5 or CVB4 vs mock infection; #p < 0.05 and ###p < 0.001 as indicated by bars; ANOVA followed by Student's t-test with Bonferroni correction. DAF, decay accelerating factor.
DOI:
http://dx.doi.org/10.7554/eLife.06990.016