Table 3.
Amount of carotenoids produced by engineered B. subtilis strains and relative increase compared to control strain
| B. subtilis strains | Total carotenoids (mg/g dcw)a | Relative increaseb |
|---|---|---|
| CrtMN | 0.60(±0.010) | 1.00 |
| p04S | 4.81(±0.114) | 8.02 |
| p04SD | 5.27(±0.140) | 8.78 |
| p04SDF | 6.83(±0.096) | 11.38 |
| p04SDFH | 9.03(±0.158) | 15.05 |
| p04C | 5.54(±0.410) | 9.23 |
| p04CE | 7.62(±0.176) | 12.70 |
| p04CEG | 7.84(±0.315) | 13.07 |
| p04GCEGA | 10.65(±0.129) | 17.75 |
aThe total amount of carotenoids was measured in triplicate (±standard deviation)
bThe relative increase is calculated as the amount of carotenoids produced in the engineered B. subtilis strain divided by the amount of carotenoids produced in the B. subtilis strain containing only the carotenoids producing plasmid pHYCrtMN